Genetic complementation by cloned bacteriophage T4 late genes
نویسندگان
چکیده
منابع مشابه
Intragenic complementation by gene 42 amber mutations of bacteriophage T4.
Phage T4 amber mutants defective in gene 42 (dCMP hydroxymethylase) were shown by in vivo and in vitro experiments to participate in both positive and negative intragenic complementation. This argues that incomplete polypeptide chains can participate in subunit interaction.
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Chimeric plasmids containing the uvsY uvsW region of the T4 genome were examined for the expression of these genes. Certain of these plasmids were shown to express the uvsY or the uvsW gene products by their ability to complement the UV sensitivity of infecting uvsW or uvsY mutant phage. Also, a chimeric plasmid containing both the uvsW and uvsY genes increases the survival of UV-irradiated, me...
متن کاملDefining a bacteriophage T4 late promoter: bacteriophage T4 gene 55 protein suffices for directing late promoter recognition.
The RNA polymerase from bacteriophage T4-infected Escherichia coli, which specifically initiates transcription at phage T4 late promoters, is extensively modified by ADP-ribosylation of core subunits and by binding several virus-encoded subunits. We show here that one of these subunits, the phage T4 gene 55 protein, designated gp55, alone endows unmodified RNA polymerase core enzyme from uninfe...
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We have developed an in vitro complementation assay for six T4 bacteriophage gene products believed to be components of the T4 DNA replication apparatus. This assay is based upon the fact that DNA synthesis in an infected cell lysate that lacks a given gene product is specifically stimulated by addition of the missing product. By the use of such an assay, two proteins that appear to be the prod...
متن کاملNucleotide sequences of bacteriophage T4 genes 9, 10 and 11.
He have represented the DNA sequences of bacter iophage T4 genes 9,10 and 11 which encode the s t r u c t u r a l p r o t e i n s of the base p l a t e ( l , 2 ) . This region has a s i n g l e T4 l a t e promoter TATAA&T& sequence (underl ined) loca ted before of gene 9 . The overlapping of terminat ion codone of preceding gene with ATG codone of next gene in t h i s region and a l s o downstr...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1981
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.39.1.31-45.1981